Fig. 5. Specificity and sensitivity of archaeal PCR amplification of a 635-bp product from cloned small subunit rDNA of Halobacterium salinarum (pHs16S) after 35 cycles. Lanes 1 and 10, 100-bp marker; lane 2, negative PCR control; lane 3, 107 copies of pHs16S; lane 4, 106 copies of pHs16S; lane 5, 105 copies of pHs16S; lane 6, 104 copies of pHs16S; lane 7, 103 copies of pHs16S; lane 8, 102 copies of pHs16S; lane 9, 2.2 × 108 copies of pEc16S.