Video 3.
Spot-tracking analysis of AC invadopodia lifetimes. Ventral-view time-lapses show a wild-type (top) and unc-60a RNAi-treated animal (bottom) just before the normal time of basement membrane breaching in wild-type animals. F-actin (mCherry::moeABD) is shown in green and laminin (laminin::GFP) is shown in magenta; spots (cyan) are overlaid on fluorescence (left) and shown alone (right). Time points were acquired every 15 s and the video plays at a rate of 10 frames per second. Bar, 5 µm. This video corresponds to Fig. 2 D.