Video 4.
FLIP analysis reveals dynamic recycling of invadopodial membrane through the endolysosomal compartment. Lateral-view time-lapses show spectral representation of fluorescence intensity of LMP-1::GFP in wild-type (left) and unc-60a RNAi-treated (right) ACs. Small 1-µm regions of the invasive membrane (circles) were continuously photobleached using 100% laser power and 20 iterations. The first time-point shown is immediately before the start of photobleaching. This analysis was pre-formed using a confocal microscope (LSM 510; Carl Zeiss) equipped with a 100× objective. 10-min time-lapses are shown with time-points acquired every 30 s. The video plays at 10 frames per second. Bar, 5 µm. This video corresponds to Fig. 5 A.