Table 4. qRT-PCR analysis of the effects of mAsh1 RNAi on expression of selected genes
mRNA |
RNAi Exp 1 |
RNAi Exp 2 |
Mouse achaetescute homolog 1 (mAsh1) |
2.56 ± 0.27 |
2.50 ± 0.16 |
Adenylyl cyclase 9 (Adcy9) |
1.71 ± 0.11 |
1.70 ± 0.07 |
Budding uninhibited by benzimidazoles 1 homolog (Bub1) |
1.31 ± 0.13 |
1.28 ± 0.06 |
Cyclin-dependent kinase inhibitor 2D (Cdkn2d/p19) |
1.30 ± 0.01 |
1.45 ± 0.21 |
Hes6 |
1.56 ± 0.04 |
1.59 ± 0.01 |
Islet amyloid polypeptide (Iapp1) |
1.45 ± 0.03 |
1.47 ± 0.08 |
N-myc downstream regulated 4 (Ndrg4) |
1.26 ± 0.01 |
1.96 ± 0.24 |
c-Ret |
1.78 ± 0.28 |
1.67 ± 0.09 |
Dopa decarboxylase (Ddc) |
1.46 ± 0.01 |
1.48 ± 0.13 |
Proto-oncoprotein c-Myb |
1.53 ± 0.15 |
1.72 ± 0.13 |
Mesoderm development candidate 2 (Mesdc2) |
1.72 ± 0.16 |
1.89 ± 0.25 |
c-Jun |
+1.30 ± 0.01 |
+1.58 ± 0.05 |
Mean values ±1 SD of data from triplicate assays of two cDNAs each independently generated from a given RNA preparation (n = two separate RNAi experiments). The concentration of each mRNA from each experiment was first normalized to Gapdh mRNA, and the fold changes were then computed by using the normalized values (D D CT method).