A Targeted Quantitative Proteomics Strategy for Global Kinome Profiling of Cancer Cells and Tissues

Supplemental Data

Files in this Data Supplement:

  • Supplementary Methods and Figures - Supplementary Methods and Figures
  • Human kinome library - Targeted kinases and kinase peptides in human MRM kinome library
  • Table S2 - A list of identified peptides as well as kinases in human MRM kinome assay for IMR-90 cell lysates.
  • Table S3 - A list of quantified peptides as well as kinases in human MRM kinome assay for protein lystates of WM-115 and WM-266-4 cells.
  • Table S4 - A list of quantified peptides as well as kinases in human MRM kinome assay for protein extracts of human lung tissue.
  • Skyline file for MRM library - MCP-kinase library-skyline.zip file is the exported Skyline document file containing the MS/MS spectra and iRT information for 386 targeted kinase peptides and 10 standard BSA peptides. To open the skyline document file, please extract MCP-kinase library-skyline.zip file into single folder and open kinase-1.sky file using Skyline software developed in MacCoss lab.