Low-Molecular-Weight Metabolites Secreted by Paenibacillus larvae as Potential Virulence Factors of American Foulbrood

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    Identified secondary metabolite gene clusters in the genomes of Paenibacillus larvae subsp. larvae B-3650 and Paenibacillus larvae subsp. larvae BRL-23001 (Table S1); larval survival after infection with different strains of P. larvae (Fig. S1); MALDI-orbitrap mass spectra of Amberlite XAD16 extract from different P. larvae subsp. larvae strains: DSM 16116, DSM 16115, DSM 17237, and NRLL B-41635 (Fig. S2); MALDI-TOF mass spectra of culture supernatants of P. larvae subsp. larvae DSM 16116, DSM 16115, and DSM 17237, all cultivated in J medium (Fig. S3); MALDI-orbitrap mass spectra of fractions obtained from C18E-SPE prepurification of the Amberlite XAD16 extract of P. larvae subsp. larvae DSM 16116 (Fig. S4); putative secondary-metabolite gene clusters identified in the genome of Paenibacillus larvae subsp. larvae DSM 25430 (Fig. S5); alignment of the primary structures of the identified lantibiotic prepropeptide in the genome of P. larvae subsp. larvae B-3650 with the primary structures of other database-deposited lantibiotic prepropeptides (Fig. S6).

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