Video 2.
Alexa Fluor 546–Ad5 motility in control siRNA-treated cells. A549 cells were infected with Alexa Fluor 546–labeled Ad5 at 10 MOI and imaged at 37 min p.i. at 17-Hz frame rate for 30 s using an epifluorescence microscope (DM IRBE; Leica). Playback is 10× speed of real time. First frame shows transmitted light channel; all subsequent frames show Alexa Fluor 546–Ad5 (100 frames, every fifth frame of the original acquisition). Cells were transfected with control siRNA and imaged 48 h later. Note the long-range motility of viral capsids especially in the top part of the cell. Video was taken at 28 min p.i. Time stamp indicates seconds after start of the video. Cell corresponds to Fig. S2 (control RNAi). Black lines depicted cell outlines.