Video 4.
Alexa Fluor 546–Ad5 motility in LIC1 wild-type rescue cells. A549 cells were infected with Alexa Fluor 546–labeled Ad5 at 10 MOI and imaged at 37 min p.i. at 17-Hz frame rate for 30 s using an epifluorescence microscope (DM IRBE; Leica). Playback is 10× speed of real time. First frame shows transmitted light channel; all subsequent frames show Alexa Fluor 546–Ad5 (100 frames, every fifth frame of the original acquisition). Cells were transfected with LIC1 siRNA 48 h before imaging and transfected with LIC1 wild-type rescue constructs 24 h before imaging. First frame shows the transmitted light channel, second frame shows GFP channel, and all subsequent frames show Alexa Fluor 546–Ad5. Note the rescue of long-range motility of several virus capsids. Video was taken at 44 min p.i. Time stamp indicates seconds after start of the video. Cell corresponds to Fig. S3 (WT rescue). Black lines depicted cell outlines.