Video 9.
Lysosome motility in Ad5-infected A549 cells treated with PKA inhibitor. Cells were preincubated with LysoTracker green dye to label lyso/LE before being processed for imaging at 2 frames/min for 90 min using an epifluorescence microscope (DM IRBE; Leica). Playback is 450× speed of real time. First frame shows a transmitted light image of the cell, and subsequent frames show LysoTracker signal (180 frames, all frames of the original acquisition). Cells were infected with 100 MOI Ad5 shortly before imaging (Materials and methods) and pretreated with the PKA inhibitor H-89 (5 µM), leading to inhibition of Ad5-induced lyso/LE dispersal. Time stamp indicates hours, minutes, and seconds after start of the video. Cell corresponds to Fig. 4 A (Ad5 + H-89). Bar, 20 µm.