Insight into Insulin Secretion from Transcriptome and Genetic Analysis of Insulin-Producing Cells of Drosophila

Files in this data supplement:

  • Supporting Information: Figures S1-S3, Files S1-S5, and Tables S1-S6 (PDF, 915 KB)
  • Figure S1: RNAi screen of IPC-enriched genes and Rab-DNs for growth phenotypes. (PDF, 603 KB)
  • Figure S2: Measurement of larval brain Ilp2 transcript levels when unc-104 mRNA was depleted or Rab1 protein function was inhibited. (PDF, 191 KB)
  • Figure S3: Expressing Rab1 RNAi in IPCs results in accumulation of ILP2 in IPCs. (PDF, 552 KB)
  • File S1: Movie of IPCs at different developmental stages (.mov, 11 MB)
  • File S2: Rotating view of larval IPCs at 93 hrs after embryo deposition (.mov, 6 MB)
  • File S3: Rotating view of pupal IPCs at 146 hrs after embryo deposition (.mov, 3 MB)
  • File S4: Rotating view of adult IPCs at 239 hrs after embryo deposition (.mov, 3 MB)
  • File S5: Supplemental Materials and Methods (PDF, 122 KB)
  • Table S1: Number of aligned mRNA reads to the D. melanogaster Refseq mRNA. (PDF, 149 KB)
  • Table S2: List of all genes with aligned reads in laser-captured IPC and control samples (.xlsx, 339 KB)
  • Table S3: List of 193 IPC-enriched genes with their annotated molecular functions (.xlsx, 18 KB)
  • Table S4: Full list of biological functional clusters annotated by DAVID with IPC-enriched transcripts (.xlsx, 87 KB)
  • Table S5: List of 109 IPC-enriched genes and their mouse orthologs (.xlsx, 11 KB)
  • Table S6: Drosophila stocks used in this study. (PDF, 137 KB)