19-Substituted Benzoquinone Ansamycin Heat Shock Protein-90 Inhibitors: Biological Activity and Decreased Off-Target Toxicity

Data Supplement

Files in this Data Supplement:

• Data Supplement -

  Supplemental Figure 1 - 19-Substitutions protect TAMH cells from 17-DMAG-induced toxicity

  Supplemental Figure 2 - Inhibition of yeast Hsp90 ATPase activity by 19-methyl BQAs

  Supplemental Figure 3 - Molecular modeling of the interactions of 19Ph-DMAG with Hsp90

  Supplemental Figure 4 - NQO1 protein expression in human breast cancer cell lines

  Supplemental Figure 5 - Immunoblot analysis of biomarkers for Hsp90 inhibition on MDA468/NQ16 and MDA468 cells treated with BQAs and 19-phenyl BQAs

• 2K5B&19phQuinone.pdb -

  Predicted protein-ligand complex consisting of Hsp90 (PDB ID: 2K5B; Chain A) and the quinone form of 19-phenyl-DMAG (Chain B)

• 2K5B&19phHydroquinone.pdb -

  Predicted protein-ligand complex consisting of Hsp90 (PDB ID: 2K5B; Chain A) and the hydroquinone form of 19-phenyl-DMAG (Chain B)