Directed Evolution of Unspecific Peroxygenase from Agrocybe aegerita

Supplemental material

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• Supplemental file 1 -

  Strategies and mutations from the directed UPO1 evolution (Table S1), mutations in the mature PaDa-I variant (Table S2), primer list (Table S3), purification and glycosylation degrees (Fig. S1), overview of all the residues mutated by evolution (Fig. S2), and supplemental materials and methods (construction of fusion genes, high-throughput dual-screening assay development, large-scale enzyme production and purification protocols, MALDI-TOF-MS analysis, pI determination, protocols for steady-state kinetic constants, pH activity profiles, and protein modeling).

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