Bacillus thuringiensis DB27 Produces Two Novel Protoxins, Cry21Fa1 and Cry21Ha1, Which Act Synergistically against Nematodes

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    Light microscopy images of B. thuringiensis DB27 cultures used in C. elegans killing assays (Fig. S1), SDS-PAGE confirmation of Cry toxin production (Fig. S2), SDS-PAGE image of Cry proteins (Fig. S3), multiple sequence alignment of three novel Cry21 protoxins discovered in this study in comparison with Cry21Ba1 toxin (Fig. S4), representative Western blot image of purified Cry21Fa1 and Cry21Ha1 proteins (Fig. S5), log-rank statistical analysis of survival curves (Table S1), potential virulence factors of B. thuringiensis DB27 detected by whole-genome sequencing (Table S2), and features of B. thuringiensis DB27 plasmids and Cry toxins detected by whole-genome sequencing (Table S3).

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