Fig 4B Fig 4C Fig 4D Fig 4E Fig 4F Fig 4G Fig 4H Fig 4I Fig 4J Time (min) phosphoMEK Time (min) phosphoERK Time (min) pERK Time (min) ppERK Time (min) nT Time (min) cpp Time (min) npp Time (min) cpp U drug expt Time (min) npp U drug expt 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3.195405188 5 1.884798999 15 2.200443059 15 2.757439196 2 0.375611747 1 0.620778917 2 0.343890338 2 0.601190223 2 0.540532578 15 1.124602202 15 2.321911059 120 0.799556941 120 0.242560804 4 0.928738565 2 1.070891492 4 0.393964263 4 1.111545058 4 0.696764391 30 0.662476138 30 1.479432939 6 1.627615176 3 1.338310584 6 0.650178291 6 1.301543179 6 0.859939598 60 0.607420163 60 0.289100558 8 2.10392968 4 1.466701209 8 0.838544653 8 1.156915933 8 0.937642043 120 0.41009631 120 0.024756444 10 2.317771543 5 1.470685058 10 0.954688717 10 1.119779741 10 1.016974443 12 2.085038107 6 1.316502599 12 0.90961688 12 1.059595045 12 1.00322178 14 1.830962621 7 1.225196466 14 1.028758095 14 1.057464237 14 0.968887851 16 1.697761756 8 1.201037363 16 1.163142752 16 1.05949956 16 1.00255854 18 1.504091971 9 1.294978322 18 1.180447416 18 1.07419368 18 0.962738141 20 1.287129375 10 1.202674913 20 1.210462622 20 1.083136557 20 0.98990787 22 1.109990568 11 1.147418072 22 1.033595775 22 1.092762253 22 1.006402396 24 1.047010824 12 1.022906664 24 1.196532204 24 1.15915302 24 1.037813781 26 0.953464579 13 1.136351918 26 1.155718663 26 1.123221514 26 1.071216397 28 1.012901113 14 1.186004689 28 1.004724019 28 0.00660806 28 1.059238455 30 0.973867104 15 1.228180628 30 1.090147422 30 -0.024736293 30 1.089597791 32 0.834776763 16 1.200488716 32 1.140745641 32 -0.061218763 32 1.195377301 34 0.889512881 17 1.178259485 34 1.214084687 34 0.019646318 34 1.204546971 36 0.797999657 18 1.163342396 36 1.11716375 36 0.076058608 36 1.139468992 38 0.713182589 19 1.051016801 38 1.128438014 38 0.100634853 38 1.213214144 40 0.841376116 20 1.155257275 40 1.158412602 40 0.176052764 40 1.270888792 42 0.811576434 21 0.967257216 42 1.096430682 42 0.180669444 42 1.264952231 44 0.873733219 22 1.039142452 44 1.174765144 44 0.346970327 44 1.203013222 46 0.687705698 23 1.008305182 46 1.222709969 46 0.369129784 46 1.265102292 48 0.854538283 24 0.835316364 48 1.096616554 48 0.324347896 48 0.269034842 50 0.784570655 25 0.977116649 50 1.100176956 50 0.362548238 50 0.133732064 52 0.702280157 26 0.938607003 52 1.085745996 52 0.346581856 52 0.013027633 54 0.771968579 27 0.989427494 54 1.179522171 54 -0.002868094 56 0.849319077 28 0.932190358 56 1.019309546 56 0.014051467 58 0.611792172 29 0.904825439 58 0.887949688 58 -0.005571577 60 0.610014293 30 0.848359597 60 1.169920076 60 -0.000896624 32 0.83624685 62 0.084634329 34 0.874610645 64 0.039584455 36 0.891691823 66 0.073108093 38 0.774237084 68 0.066186481 40 0.896125172 70 0.03665592 42 0.812260897 72 0.12949725 44 0.901031579 74 0.158376817 46 0.844707317 76 0.144129412 48 0.885722179 78 0.159909533 50 0.93611033 80 0.170980399 52 0.83718001 82 0.153037515 54 0.869717045 84 0.237586291 56 0.844823172 86 0.207406834 58 0.979396565 88 0.195203018 60 0.906895594 90 0.12101794