cutadapt parameters: -a TCGTATGCCGTCTTCTGCTTG -g AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGACGATC -b CGTACGCGGGTTTAAACGA -b CGTACGCGGAATAGTTTAAACTGT -n 1 -m 20 bowtie parameters: -v 1 -m 10 --all --best --strata PARalyzer parameters (.ini file settings): BANDWIDTH=3 CONVERSION=T>C MINIMUM_READ_COUNT_PER_GROUP=2 MINIMUM_READ_COUNT_PER_CLUSTER=2 MINIMUM_READ_COUNT_FOR_KDE=3 MINIMUM_CLUSTER_SIZE=11 MINIMUM_CONVERSION_LOCATIONS_FOR_CLUSTER=2 MINIMUM_CONVERSION_COUNT_FOR_CLUSTER=2 MINIMUM_READ_COUNT_FOR_CLUSTER_INCLUSION=1 MINIMUM_READ_LENGTH=20 MAXIMUM_NUMBER_OF_NON_CONVERSION_MISMATCHES=0 Annotation priority (.yaml file): # Comments are allowed and are indicated with the pound (aka octothorpe) symbol # Higher numeric value indicates higher priority trailing annotation: coding: aligned: show : false priority : 13 3'utr: aligned: show : false priority : 12 downstream: show : false region : 1000 5'utr: aligned: show : false priority : 11 upstream: show : false region : 1000 tss: aligned: show : false priority : 1 tes: aligned: show : false priority : 1 intron: aligned: show : false priority : 7 lincRNA: aligned: show : false priority : 6 piRNA: aligned: show : false priority : 5 miRNA: aligned: show : false priority : 8 rRNA: aligned: show : false priority : 40 snoRNA: aligned: show : false priority : 34 snRNA: aligned: show : false priority : 33 miscRNA: aligned: show : false priority : 19 Mt rRNA: aligned: show : false priority : 22 Mt tRNA: aligned: show : false priority : 21 tRNA: aligned: show : false priority : 30 repeat: aligned: show : false priority : 29