The I-TevI Nuclease and Linker Domains Contribute to the Specificity of Monomeric TALENs

Supporting Information for Kleinstiver et al., 2014

Files in this Data Supplement:

  • Supporting Information - Figures S1-S4 and Table S1 (PDF, 412 KB)
  • Figure S2 - mTALEN activity. (PDF, 161 KB)
  • Figure S3 - Screening of randomized DNA spacer library. (PDF, 191 KB)
  • Figure S4 - Example purification of an untagged Tev-mTALEN construct. (PDF, 265 KB)
  • Table S1 - mTALEN constructs, named according to the length of the I-TevI fragment and the N-terminal residue of the PthXo1 TALE domain. (PDF, 114 KB)
  • Figure S1 - Sequences of Tev-mTALENs. (.txt, 45 KB)