Supplemental Figures S1-S4 and Tables S1-S6.
Files in this Data Supplement:
Supplemental Figure S1. (A) Schematic representation of pmGENIE-3-EGFP. The locations of PCR primers are indicated by arrows. (B) Location in pmGENIE-3-EGFP to which the pB gene start and 3?-TRE Southern blot probe was constructed. (C) Location to which the EGFP Southern blot probe was constructed in pmGENIE-3-EGFP.
Supplemental Figure S2. (A) New born piglets under Bright field and Fluorescence lighting. Only one piglet demonstrated variegated EGFP expression. (B) EGFP expression of various dissected organs from a male transgenic piglet and a male wild type piglet euthanized at the age of one week.
Supplemental Figure S3. Southern blot analysis for all born transgenic pigs, done with a probe to the EGFP gene found in the pmGENIE-3-EGFP transposon. Transgenic pig TG9 is omitted in this analysis.
Supplemental Figure S4. Identification of transposon insertion sites from the four first born transgenic piglets. The pB 5?-TRE sites are flanked by the chromosomal sequences where the transposon inserted. The TTAA sequence on the border signifies transpositional integration of the transposon.
Supplemental Table S1. Primers for PCR.
Supplemental Table S2. Concentration effect of CTI delivered pmGENIE-3-EGFP plasmid on the positive rate of parthenogenetic embryos expressing EGFP.
Supplemental Table S3. Effect of cytoplasmic co-injection of pmGENIE-3-EGFP and mPB plasmids on the positive rate of embryos expressing EGFP.
Supplemental Table S4. Comparison of gene transfer efficiency between the pBt based plasmid pmGENIE-3-EGFP and the non-insertional pEGFP-N1 plasmid during CTI.
Supplemental Table S5. Effect of CTI treatment time points on the positive rate of IVF embryos expressing EGFP when injected with pmGENIE-3-EGFP.
Supplemental Table S6. Production of transgenic pigs by CTI co-injection of pmGENIE-3-EGFP and mPB plasmids into IVF derived embryos.