Leukotriene B₄ amplifies eosinophil accumulation in response to nematodes

Files in this Data Supplement:

• Supplemental Material (PDF)
• Video 1 -
  Time-lapse video of bone marrow–derived eosinophils and a C. elegans dauer larva over the course of 80 min. Images were acquired once every 30 s and are displayed at a frame rate of 15/s. Collective migration toward the debris in the top right was not apparent. Stills from this video are shown in Fig. 1. Results represent three independent experiments. Bar, 50 µm.
• Video 2 -
  Time-lapse video of bone marrow–derived eosinophils (red) and a C. elegans dauer larva (green) over the course of 60 min. Images were acquired once every 20 s and are displayed at a frame rate of 15/s. At the 54-min time point, the shifting worm carries a large aggregate of eosinophils roughly 100 µm to the right. Results represent three independent experiments. Bar, 50 µm.
• Video 3 -
  Time-lapse video of human peripheral blood eosinophils and a C. elegans dauer larva over the course of 45 min. Images were acquired once every 15 s and are displayed at a frame rate of 15/s. Results represent two independent experiments, each with cells from a different donor. Bar, 50 µm.
• Video 4 -
  Time-lapse video of control (green) and PT-treated (red) bone marrow–derived eosinophils and a C. elegans dauer larva (blue) over the course of 120 min. Images were acquired once every 60 s and are displayed at a frame rate of 10/s. Fluorescent eosinophils and invisible nonfluorescent control-treated eosinophils make up 30% and 70% of the total cells, respectively. Stills from this video are shown in Fig. 2. Results represent three independent experiments. Bar, 50 µm.
• Video 5 -
  Time-lapse video of Alox5^+/+ (green) and Alox5^−/− (red) bone marrow–derived eosinophils and a C. elegans dauer larva (blue) over the course of 60 min. Images were acquired once every 60 s and are displayed at a frame rate of 10/s. Fluorescent eosinophils and invisible nonfluorescent Alox5^+/+ eosinophils make up 30% and 70% of the total cells, respectively. Stills from this video are shown in Fig. 3. Results represent two independent experiments. Bar, 50 µm.