Table 1.
Cross-reaction of mouse antiserum for 6-keto-PGF1α with other prostanoids and arachidonic acid
| Compound | Cross-reaction (%)a |
|---|---|
| 6-Keto-PGF1α | 100 |
| PGF2α | 1.5 |
| PGE2 | 0.1 |
| PGA2 | <0.1 |
| PGB2 | <0.1 |
| PGD2 | <0.1 |
| 11-β-PGF2α | <0.1 |
| 8-iso-PGF2α | <0.1 |
| Arachidonic acid | <0.1 |
| Thromboxane B2 | <0.1 |
aELISA was performed under the standard conditions for 6-keto-PGF1α in the presence of increasing amounts of prostanoids and free arachidonic acid. The amount of each compound to be tested for 50 % binding was compared with that of standard 6-keto-PGF1α