Streptococcus pyogenes Polymyxin B-Resistant Mutants Display Enhanced ExPortal Integrity

Supplemental material

Files in this Data Supplement:

• Supplemental file 2 -

  Fig. S1, use of protease indicator plates to monitor SpeB expression

  Table S1, bacterial strains containing engineered and complemented mutations

  Table S2, auxotrophic P_i revertant mutants isolated in this study

  Table S3, plasmids

  Table S4, mutagenesis and complementation primers

  Table S5, real-time RT-PCR primers

  Table S7, transitions and transversions of SNPs

  Table S8, number of deleted or inserted nucleotides in indels

  Table S9, deletions or insertions of repetitive elements

  Table S10, distribution of mutation type in polymyxin B-resistant isolates

  Table S11, distribution of core polymyxin B resistance gene mutations isolated from independent experiments

  Table S12, genomic analysis of S. pyogenes HSC5 sugar transporters

  PDF, 3.0M

• Supplemental file 1 -

  Table S6, whole-genome resequencing data for polymyxin B-resistant mutants

  XLSX, 26K