Leduc et al. 10.1073/pnas.0401365101.

Supporting Information

Files in this Data Supplement:

Supporting Table 3

Table 3. Plasmids and DNA oligonucleotides described in this work

	Mutation(s)
Plasmids	Modified codon	Amino acid replacement	Mutagenic DNA oligonucleotides*
pGL2^†	–	–	–
pHP1	CGG>GCG	R74A	TTGCAAGAATTGAGCGCGCATAGAATAGCGAGC (1)
pHP2	CGG>AAG	R74K	TTGCAAGAATTGAGCAAGCATAGAATAGCGAGC (2)
pHP3	TCA>GCT	S84A	GCGAGCTTGAGCGTGAAAGCTAGCCGTTACACTTTGAGG (3)
pHP4	TCA>TGC	S84C	GCGAGCTTGAGCGTGAAATGCAGCCGTTACACTTTGAGG (4)
pHP5	TCA>TAT	S84Y	GCGAGCTTGAGCGTGAAATATAGCCGTTACACTTTGAGG (5)
pHP6	AGC>GCC	S85A	AGCTTGAGCGTGAAATCAGCCCGTTACACTTTGAGGG (6)
pHP3/6	TCA>GCT/AGC>GCC	S84A/S85A	Oligos (3) and (6) were used
pHP7	TAC>TTT	Y87F	GCGTGAAATCAAGCCGTTTTACTTTGAGGGAATTG (7)
pHP8	CAT>CAA	H48Q	GGGAATATTTTTAGGCAATCTTCCCACTTTAGAG (8)
pHP8/3	CAT>CAA/TCA>GCT	H48Q/S84A	Oligos (3) and (8) were used
pHP8/4	CAT>CAA/TCA>TGC	H48Q/S84C	Oligos (4) and (8) were used

Bold type in DNA oligonucleotides refers to the mutation introduced.

*Polarity of shown DNA sequences is from 5′ to 3′.

^{†pGL2 carries a wild-type allele of H. pylori thyX that complements in an arabinose-dependent manner E. coli strain χ 2913 (thyA) to thymidine prototrophy (1). This plasmid was used as template for the site-directed mutagenesis reactions to obtain the mutant alleles described.

1. Myllykallio, H., Lipowski, G., Leduc, D., Filee, J., Forterre, P. & Liebl, U. (2002) Science 297, 105–107.}