Table 1. Deletion sizes (bp) in the rescued clones
|
Young |
Presenescent |
Senescent |
||||
|
No. |
HindIII |
I-SceI |
HindIII |
I-SceI |
HindIII |
I-SceI |
|
1 |
0 |
0 |
0 |
0 |
0 |
1 |
|
2 |
0 |
1 |
0 |
1 |
0 |
2 |
|
3 |
0 |
2 |
0 |
1 |
0 |
4 |
|
4 |
0 |
2 |
0 |
2 |
0 |
4 |
|
5 |
0 |
3 |
0 |
3 |
0 |
4 |
|
6 |
0 |
4 |
0 |
4 |
0 |
6 |
|
7 |
0 |
4 |
0 |
5 |
0 |
7 |
|
8 |
0 |
4 |
10 |
5 |
0 |
8 |
|
9 |
0 |
5 |
48 |
7 |
0 |
8 |
|
10 |
0 |
5 |
50 |
8 |
0 |
8 |
|
11 |
0 |
6 |
60 |
9 |
0 |
10 |
|
12 |
0 |
7 |
110 |
14 |
0 |
10 |
|
13 |
0 |
7 |
230 |
66 |
0 |
12 |
|
14 |
0 |
8 |
667 |
208 |
10 |
20 |
|
15 |
0 |
8 |
824 |
250 |
20 |
25 |
|
16 |
0 |
9 |
914 |
374 |
35 |
38 |
|
17 |
0 |
10 |
917 |
500 |
50 |
50 |
|
18 |
0 |
13 |
1,074 |
544 |
70 |
70 |
|
19 |
6 |
15 |
1,083 |
685 |
73 |
160 |
|
20 |
15 |
18 |
1,100 |
1,004 |
80 |
244 |
|
21 |
19 |
20 |
1,113 |
1,300 |
83 |
270 |
|
22 |
21 |
39 |
1,120 |
1,427 |
331 |
330 |
|
23 |
28 |
70 |
1,195 |
1,528 |
400 |
351 |
|
24 |
30 |
103 |
1,400 |
1,600 |
603 |
387 |
|
25 |
40 |
540 |
1,528 |
2,000 |
1,050 |
500 |
|
26 |
50 |
730 |
1,750 |
2,100 |
1,100 |
562 |
|
27 |
54 |
1,395 |
1,800 |
2,283 |
1,227 |
671 |
|
28 |
57 |
1,447 |
1,848 |
2,463 |
1,274 |
1,327 |
|
29 |
70 |
1,583 |
1,900 |
2,529 |
1,343 |
1,443 |
|
30 |
80 |
1,640 |
2,024 |
2,642 |
1,400 |
1,479 |
|
31 |
90 |
1,653 |
2,149 |
2,700 |
1,639 |
1,600 |
|
32 |
104 |
1,800 |
2,202 |
2,800 |
1,864 |
1,650 |
|
33 |
177 |
1,872 |
2,258 |
2,939 |
1,900 |
1,754 |
|
34 |
1,148 |
2,321 |
2,952 |
2,025 |
1,900 |
|
|
35 |
1,473 |
2,442 |
4,874 |
2,431 |
2,000 |
|
|
36 |
1,894 |
2,733 |
4,937 |
2,863 |
2,076 |
|
|
37 |
1,970 |
3,450 |
3,275 |
3,200 |
||
|
38 |
2,100 |
3,587 |
3,473 |
|||
|
39 |
4,862 |
3,775 |
||||
|
Median* |
10 |
1,074 |
83 |
|||
|
Average* |
316 |
1,247 |
718 |
|||
Deletion size was calculated as a sum of deletions from both ends.
*Median and average was calculated from a combined data set for HindIII and I-SceI clones.