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Grieshaber et al. 10.1073/pnas 0400754101. |
Fig. 6. Schematic of plasmid constructs designed for genetic screen. The genetic screen was carried out as described in Materials and Methods.
Fig. 7. RT-PCR of C. trachomatis L2 MEP pathway genes. RT-PCR was carried out as previously described (1). euo, ompA, and hctA are examples of early (2 h), mid-cycle (12 h), and late (20 h) genes. UI indicates uninfected HeLa cells and C (control) indicates C. trachomatis L2 genomic DNA.
Fig. 8. Matrix-assisted laser desorption ionization--time-of-flight mass spectroscopy of Hc1 from chlamydial nucleoid treated with deproteinated extract from E. coli expressing pLac (A) or CT804 (B). Peaks at 13,566 Da represent Hc1. (C) DNase I digestion of C. trachomatis L2 EB nucleoids incubated with deproteinated extracts of E. coli cultures expressing pLac or CT804.
1. Shaw, E. I., Dooley, C. A., Fischer, E. R., Scidmore, M. A., Fields, K. A. & Hackstadt, T. (2000) Mol. Microbiol. 37, 913-925.