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Fig. 8. Effect of cAMP-dependent protein kinase (PKA) agonists on [¹⁴C]urea uptake by oocytes expressing UT-A1, UT-A1^(10–), UT-A3, and UT-A3^(10–). Open bars indicate urea uptake under control conditions; filled bars indicate urea uptake after the preincubation of oocytes for 1 h in 0.5 mM 8-bromoadenosine 3',5'-cyclic monophosphate, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX), and 0.05 mM forskolin; and hatched bars indicate urea uptake after preincubation of oocytes with 0.5 mM phloretin. Values are mean ± SE; n = 6. *, Significant increase in urea uptake compared with the H₂O-injected control group (P < 0.05). y , Significant increase in uptake caused by PKA agonists compared with the paired control group (P < 0.05). j , Significant decrease in uptake by 0.5 mM phloretin compared with the paired group (P < 0.05). (A) Summary of the urea accumulated in a 90-s period by H₂O-injected control oocytes or oocytes expressing UT-A1 or UT-A1^(10–) and the effects of cAMP agonists. (B) Summary of the urea accumulated in a 90-s period by H₂O-injected control oocytes or oocytes expressing UT-A1 or UT-A3^(10–) and the effects of cAMP agonists. (C) Summary of the urea accumulated in a 90-s period by H₂O-injected control oocytes or oocytes expressing UT-A1 or UT-A3^(10–) and the effects of 0.5 mM phloretin.