A sterol-enriched vacuolar microdomain mediates stationary phase lipophagy in budding yeast

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  Localization of LDs in wild-type cells during stat-phase. Wild-type cells stained with FM4-64 (vacuole) at DS and with BODIPY (LDs) at the indicated time points. Images were analyzed by time-lapse microscopy using the Delta Vision system (Applied Precision). Frames were taken every 30 s for 30 min. Cells in D4 were also imaged for z sections. Frames from top to bottom were taken every 0.2 µm for 5 µm. The dotted circles outline the boundaries of yeast cells. N, nucleus; V, vacuole.