Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1

Files in this Data Supplement:

• Supplemental Material (PDF)
• Video 1 -
  CLAMP dynamically interacts with MTs. Representative FRAP experiment of an RPE-1 cell transfected with hCLAMP-GFP as quantified in Fig. 4 E. Images were collected using laser-scanning time-lapse confocal microscopy (A1R; Nikon). Frames were collected every 2 s.
• Video 2 -
  CLAMP rapidly diffuses throughout the cytoplasm. Movie of a photoactivation experiment in an RPE cell transfected with PA-GFP-hCLAMP shown in Fig. 4 F. Images were collected using laser-scanning time-lapse confocal microscopy (A1R; Nikon). Frames were collected every 2 s.