A Novel Protein, RafX, Is Important for Common Cell Wall Polysaccharide Biosynthesis in Streptococcus pneumoniae: Implications for Bacterial Virulence

Supplemental material

Files in this Data Supplement:

• Supplemental file 4 -

  Fig. S1, impact of rafX gene deletion on WTA-PG banding pattern

  Fig. S2, conservation of rafX gene among clinically isolated strains

  Fig. S3, banding pattern of WTA-PG from R6 ΔrafX mutant following digestion with enzymes

  Fig. S4, alignment of S. pneumoniae RafX with P. aeruginosa PAO1 WaaL using BLAST database

  Fig. S5, growth curves of bacteria grown in the indicated media

  Fig. S6, LytA expression was not influenced by rafX deletion

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• Supplemental file 1 -

  Table S1, strains and plasmids

  XLS, 34K

• Supplemental file 2 -

  Table S2, primers

  XLS, 26K

• Supplemental file 3 -

  Table S3, clinical isolates

  XLSX, 13K