Functional Metabolic Map of Faecalibacterium prausnitzii, a Beneficial Human Gut Microbe

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Supplemental results

  Fig. S1, F. prausnitzii growth on chemically defined medium

  Fig. S2, subsystem participation of predicted essential genes for iFpraus_v1.0 on simulated glucose minimal medium

  Fig. S3, pathway utilization in F. prausnitzii’s central metabolism predicted by iFpraus_v1.0

  Fig. S4, shadow prices computed for a phenotypic phase plane analysis performed for iFpraus_v1.0

  Table S1, relative growth rates on known carbon sources with glucose as a reference, determined for iFpraus_v1.0 in silico and for F. prausnitzii A2-165 in vivo

  Table S4, secretion products that may be produced by F. prausnitzii A2-165 in vitro, and model capabilities to synthesize them before and after curation

  Table S5, allowed flux spans of secretion products calculated in silico for iFpraus_v1.0 on anaerobic glucose medium and aerobic glucose medium

  Table S6, relation between flux through flavin reductase and biomass production predicted by iFpraus_v1.0

  Table S8, simulation constraints for glucose minimal medium

  Table S9, simulation constraints for YCFA medium

  Table S10, simulation constraints for CDM2

  Table S11, composition of CDM1

  Table S12, composition of CDM2

  Table S13, composition of CDM3

  PDF, 1.6M

• Supplemental file 2 -

  Table S2, predicted biomass precursor auxotrophy and prototrophy and utilization as nitrogen and sulfur sources for F. prausnitzii A2-165

  XLSX, 21K

• Supplemental file 3 -

  Table S3, comparison of assigned functions for all genes included in the reconstruction

  XLSX, 71K

• Supplemental file 4 -

  Table S7, description of reactions and metabolites included in the final reconstruction iFpraus_v1.0

  XLSX, 162K

• Supplemental file 5 -

  Model in sbml format

  XLSX, 311K