Biosynthesis of the β-Methylarginine Residue of Peptidyl Nucleoside Arginomycin in Streptomyces arginensis NRRL 15941

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  Primers used in this study (Table S1), deduced functions of ORFs in the arginomycin biosynthetic gene cluster (Table S2), strains and plasmids used in this study (Table S3), analysis of TyrB function in the reaction (Table S4), CGA synthase gene fragment selected as the probe (Fig. S1), amplification of putative CGA synthase gene fragment (Fig. S2), heterologous production of arginomycin in S. coelicor M145 confirmed by MS analysis (Fig. S3), HR-ESI-MS analyses (Fig. S4, S5, and S9), substrate flexibility of ArgM (Fig. S6), construction and confirmation of vector pFJ7 and SDS-PAGE (Fig. S7), MS and MS/MS analysis of DNS-Arg and DNS-?-methyl Arg (Fig. S8), survey of amino donors for the ?-methylargine formation (Fig. S10), and ¹H NMR (Fig. S11), ¹³C NMR (Fig. S12), ¹H?¹H COSY (Fig. S13), and HSQC (Fig. S14) spectra of DNS-β-methylarginine.

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