Sensitive and Specific Enzyme-Linked Immunosorbent Assay for Detecting Serum Antibodies against Mycobacterium avium subsp. paratuberculosis in Fallow Deer

Supplemental material

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  • Supplemental file 1 -

    Table S1. Mean OD readings for each serum and HRP-conjugated anti-fallow deer IgG dilution. Fig. S1. Optimization of the concentrations of serum samples and anti-fallow deer IgG conjugate that provide the best signal-to-noise ratios. Fig. S2. Dynamics of sensitivity, specificity, semi-sum of sensitivity and specificity or diagnostic value, specificity discriminating index, and sensitivity discriminating index of the ELISAs A, B, C, D, and E.

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