Improved Production of a Heterologous Amylase in Saccharomyces cerevisiae by Inverse Metabolic Engineering

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 -

    Effects of the plasmids extracted from the mutation strains on cell growth and amylase production (Fig. S1); Venn diagrams showing significantly changed genes between different strains in the transcriptome experiment (Fig. S2); procedure for site-directed point mutation (Fig. S3); effect of Vta1S196I mutation on periplasmic invertase production in shake flask fermentation (Fig. S4); overall statistics of the whole-genome Illumina sequencing (Table S1); primers used for amplification of fragments to apply point mutation (Table S2); Delft medium (defined minimal medium) for shake flask cultivation (Text S1).

    PDF, 276K

  • Supplemental file 2 -

    Transcriptional responses of all the genes in M715 and M1052 compared to AAC (Data Set S1).

    XLSX, 481K

  • Supplemental file 3 -

    Single nucleotide mutations in coding regions (Data Set S2).

    XLSX, 567K

  • Supplemental file 4 -

    INDELs in upstream regions (Data Set S3).

    XLSX, 75K

  • Supplemental file 5 -

    Single nucleotide mutations in upstream regions (Data Set S4).

    XLSX, 75K