Improved Production of a Heterologous Amylase in Saccharomyces cerevisiae by Inverse Metabolic Engineering

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Effects of the plasmids extracted from the mutation strains on cell growth and amylase production (Fig. S1); Venn diagrams showing significantly changed genes between different strains in the transcriptome experiment (Fig. S2); procedure for site-directed point mutation (Fig. S3); effect of Vta1S196I mutation on periplasmic invertase production in shake flask fermentation (Fig. S4); overall statistics of the whole-genome Illumina sequencing (Table S1); primers used for amplification of fragments to apply point mutation (Table S2); Delft medium (defined minimal medium) for shake flask cultivation (Text S1).

  PDF, 276K

• Supplemental file 2 -

  Transcriptional responses of all the genes in M715 and M1052 compared to AAC (Data Set S1).

  XLSX, 481K

• Supplemental file 3 -

  Single nucleotide mutations in coding regions (Data Set S2).

  XLSX, 567K

• Supplemental file 4 -

  INDELs in upstream regions (Data Set S3).

  XLSX, 75K

• Supplemental file 5 -

  Single nucleotide mutations in upstream regions (Data Set S4).

  XLSX, 75K