Iron Triggers λSo Prophage Induction and Release of Extracellular DNA in Shewanella oneidensis MR-1 Biofilms

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  Bacterial strains and plasmids (Table S1) and oligonucleotides (Table S2) used in this study, Western immunodetection of phage λSo in in-frame deletion strains and complementation strains in the absence and presence of H₂O₂ (Fig. S1), SDS-PAGE loading controls for λSo Western immunodetection assays (Fig. S2), λSo prophage-mediated lysis is required for normal biofilm formation (Fig. S3), genetic organization of prophage λSo and site-specific transcriptional fusion constructs (Fig. S4), characterization of H₂O₂-resistant mutants OxyR^T104N and OxyR^L197P (Fig. S5), Western immunodetection of phage λSo in the wild type and a wild-type strain carrying plasmid pBBR1-TT-Ptac-MCS5-sodB for constitutive overexpression of the sodB gene (SO_2881) in the absence or presence of 0.2 mM/1 mM paraquat (Fig. S6), Western immunodetection of phage λSo in biofilm cells of the wild type and the OxyR^T104N and OxyR^L197P mutant strains cultivated under static conditions in LM medium without and with 20 μM additional Fe²⁺ (Fig. S7), and activity of promoter P_tonB and P_dps during biofilm development (Fig. S8).

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