Files in this Data Supplement:
Fig. S1. Micrograph of lung tissue prior to preparation. Fig. S2. Schematic of the experiment, showing numbers of cubes dissected from lungs and assigned to different treatments/assays. Fig. S3. Example of lung cubes after 24 hours of incubation at 37°C in ASM plus washing in PBS. Fig. S4. Gram-stained sections of tissue infected with WT PAO1 (a) and PAO1 lasR::Gm (b), viewed at 1,000× magnification. Fig. S5. Number of CFU of P. aeruginosa recovered after 24 hours of incubation in artificial sputum medium, with pig lung cube replaced with a corresponding volume of ASM.
PDF, 1.6M