Triggering TLR2, -3, -4, -5, and -8 Reinforces the Restrictive Nature of M1- and M2-Polarized Macrophages to HIV

Supplemental material

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  Table S1 (Polarization and priming of macrophages with the various TLR agonists did not affect cell surface expression of CD4 and CCR5.)

  Fig. S1 (M1 polarization and triggering TLR2, -3, -4, and -8 rendered MDMs poorly permissive to HIV infection.)

  Fig. S2 (TLR triggering reinforces the low permissiveness of polarized macrophages.)

  Fig. S3 (Macrophages lost no viability for 7 days when cultured with the distinct TLR agonists as indicated.)

  Fig. S4 (M1-polarized macrophages had increased reactivity in response to LPS or 3M-001.)

  Fig. S5 (M1- and M2-polarized macrophages and MDMs treated with poly(I·C), LPS, flagellin, 3M-001, or 3M-002 were subjected to gene profiling.)

  Fig. S6 (The antibody to IFN-αR was highly efficient in blocking the IFN pathway.)

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