Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Supplemental Movie S1 GFP-RabE carriers arriving at the prominent SPK in a cell undergoing apical extension. The GFP channel is shown in inverted grey scale. Strain MAD4120. Time is in min:sec:msec.
- Movie02 - Supplemental Movie S2 mCherry-RabE colocalizing with MyoE-GFP in the SPK of several hyphae growing over a ∼ 30 min period. Strain MAD4403 cultured on fructose. Time is in h:min:sec
- Movie03 - Supplemental Movie S3 Late Golgi cisternae labeled with mRFP-PHOSBP mature into GFP-RabE post-Golgi carriers over time. These carriers are delivered to the SPK. Individual frames represent maximal intensity projections of deconvolved z-stacks of strain MAD4440 (1 stack every 4 sec). Time is in min:sec.
- Movie04 - Supplemental Movie S4 Late Golgi cisternae labeled with mRFP-PHOSBP co-filmed with GFP-RabE with a time resolution of 250 msec. The boxed region contains two LGC captured during their transition to RabERAB11 carriers that subsequently undergo movement towards the SPK. Frames represent middle planes of strain MAD4440. Time is in sec:msec
- Movie05 - Supplemental Movie S5 Late Golgi cisternae labeled with HypBSec7-GFP co-filmed with mCherry-RabE. Individual frames represent maximal intensity projections of deconvolved Z-stacks of strain MAD5098 (1 stack every 3.3 sec). An example of a LGC that forms after 9 sec and matures into a RabE carrier is arrowed in the corresponding channels. Time is in min:sec.
- Movie06 - Supplemental Movie S6 GFP-RabE carriers following trajectories of TubA-GFP MTs. An example of one such carrier moving to the SPK is indicated with a grey arrowhead. Strain MAD4263. Time is in sec:msec.
- Movie07 - Supplemental Movie S7 Movie highlighting one example of a GFP-RabE carrier (grey arrowhead) that moves basipetally within the tip region, following the trajectory of a TubA-GFP MT in strain MAD4263. Time is in sec:msec
- Movie08 - Supplemental Movie S8 Rapidly moving GFP-RabE structures in a hyphal tip of strain MAD4120 cell treated with latB. Time is in sec:msec
- Movie09 - Supplemental Movie S9 GFP-RabE structures rapidly moving on the MT conveyor belt in latB-treated MAD4263 cell that co-expresses GFP-labeled TubA to reveal the position of the MTs. Time is in sec:msec.
- Movie10 - Supplemental Movie S10 GFP-RabE carriers arriving at the characteristic apical crescents of several myoEΔ hyphal tip cells. Strain MAD4409. Time is in sec:msec.
- Movie11 - Supplemental Movie S11 Two parallel sequences with the same time resolution of GFP-RabE carriers showing decoration of actin cables in a myoEΔ tip (top sequence) alongside with a benomyl-treated myoEΔ tip (bottom). Note the complete absence of moving structures and actin cable decoration in the bottom sequence. Strain MAD4409. Time in sec:msec.