The Temperature Gradient-Forming Device, an Accessory Unit for Normal Light Microscopes To Study the Biology of Hyperthermophilic Microorganisms

Supplemental material

Files in this Data Supplement:

  • Supplemental file 1 -

    Supplemental movie legends, comparison of swimming speeds for 15 different Thermococcus species (Fig. S1), thermotaxis behavior of Thermococcus stetteri in the TGFD (Fig. S2), and comparison of different cell appendages (Fig. S3).

    PDF, 491K

  • Supplemental file 2 -

    Reaction of Methanocaldococcus villosus to a temperature shift from room temperature to 90°C (Movie S1).

    AVI, 19M

  • Supplemental file 3 -

    Reaction of Methanocaldococcus villosus to a temperature shift from room temperature to 90°C, after 6 months of “cold ageing” at 6°C (Movie S2).

    AVI, 15M

  • Supplemental file 4 -

    Reaction of Methanocaldococcus villosus cells analyzed in Movie S2 to a temperature gradient from 90 to 140°C (Movie S3).

    AVI, 17M