Dynamin1 Is a Novel Target for IRSp53 Protein and Works with Mammalian Enabled (Mena) Protein and Eps8 to Regulate Filopodial Dynamics

Supplemental Data

Files in this Data Supplement:

• Video legends (.pdf, 7 KB) - Legends for videos.
• Video1 (.avi, 933 KB) - Video1 shows filopodial dynamics of N1E-115 cells transfected with cDNA encoding for mCherrylifeact and GFP using time-lapse microscopy.
• Video2 (.avi, 656 KB) - Video2 shows filopodial dynamics of N1E-115 cells transfected with cDNA encoding for mCherrylifeact and GFP-IRSp53 using time-lapse microscopy.
• Video3 (.avi, 857 KB) - Video3 shows filopodial dynamics of N1E-115 cells transfected with cDNA encoding for mCherrylifeact and Dyn1-GFP using time-lapse microscopy.
• Video4 (.avi, 1.3 MB) - Video4 shows filopodial dynamics of N1E-115 cells transfected with cDNA encoding for mCherrylifeact and GFP-Mena using time-lapse microscopy.
• Video5 (.avi, 1.1 MB) - Video5 shows persistent filopodia and increased filopodial lifetime of N1E-115 cells over-expressing mCherry-lifeact and GFP-Mena using time-lapse microscopy.
• Video6 (.avi, 1.4 MB) - Video6 shows filopodial dynamics of N1E-115 cells transfected with cDNA encoding for mCherrylifeact and GFP-Eps8 using time-lapse microscopy.