Files in this Data Supplement:
Supplemental Materials and Methods (general analytical procedures, size exclusion chromatography, dynamic light scattering, CD measurements, one-dimensional NMR spectra, EMSA); genetic organization of the aio locus (Fig. S1); analysis of the purified recombinant AioF-His tag produced in Escherichia coli (Fig. S2); analysis of the structure of the purified recombinant AioF-His tag-As(III) produced in Escherichia coli (Fig. S3); effect of heating on the purified recombinant AioF-His tag-As(III) protein, analyzed by MALDI-TOF mass spectrometry (Fig. S4); binding of AioF-His tag loaded with As on the aioX-aioB intergenic region, analyzed by gel mobility shift assays (Fig. S5); analysis of the AioF-His tag-As(III) binding on different regions of the aioX-aioB intergenic region by gel mobility shift assays (Fig. S6); multiple alignment of the AioF protein homologs (Fig. S7); oligonucleotides used in this study (Table S1); taxonomic distribution of the AioF homologs with their structural and functional features (location of the HTH and CXXXC motifs) (Table S2).
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