Files in this Data Supplement:
Comparison of the fragments obtained from MS/MS of the 3,234.48-Da peptide and the theoretical mass of predicted subtilomycin (Table S1); location of ApnI-like immunity protein-encoding genes in the genome of the corresponding producer strains (Table S2); MALDI-TOF mass spectral detection of subtilomycin from crude extracts of wild-type strain BSn5 and mutants (Fig. S1); detection the production of subtilomycin from the supernatant of the recombinant with subtilomycin cluster integrated into the B. subtilis CU1065 chromosome by high-resolution LC-ESI-MS (Fig. S2); detection of paenibacillin production in crude extracts of Paenibacillus ATCC 842 by high-resolution LC-ESI-MS (Fig. S3); preparative reversed-phase HPLC purification of the subtilomycin present in ammonium sulfate crude extracts from 12-h LB cultures of BSn5 (Fig. S4).
PDF, 799K