Comprehensive High-Throughput RNA Sequencing Analysis Reveals Contamination of Multiple Nasopharyngeal Carcinoma Cell Lines with HeLa Cell Genomes

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Fig. S1 to Fig. S6 (RNA CoMPASS analyses of c666-1, x666-1, CNE1, HONE1, HK1, and NP460.)

  Fig. S7 (MuLV detected in c666-1, x666-1, and NP460 cell lines.)

  Fig. S8 (HPV-18 detected in three independent sources of CNE1 cell lines.)

  Fig. S9 (RNA CoMPASS analysis of CNE1-JPN.)

  Fig. S10 (Strand-specific RNA-sequencing of ribodepleted RNA from CNE1, CNE1-JPN, and HONE1 cell lines.)

  Fig. S11 to S15 (HPV-18 breakpoints 1-a, 2-c, 3-d, 4-e, and 5-b.)

  Fig. S16 (HPV-18 SNV analysis shows identical SNVs in HeLa, CNE1, and HONE1 cells.)

  Fig. S17 (Specific SNVs within CNE1 and HONE1 exhibit heterogeneity relative to HeLa cells.)

  Table S1 (PCR primers.)

  Table S2 (Human viruses used in custom viral genome.)

  Table S3 (Quantification of HPV-18 genes in NPC cell lines.)

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