Antibodies to PhnD Inhibit Staphylococcal Biofilms

Supplemental material

Files in this Data Supplement:

• Supplemental file 1 -

  Table S1. Strains and plasmids. Fig. S1. PhnD antibodies inhibit S. epidermidis biofilm formation in the standard microtiter plate assay. Legends to Movies S1 to S7.

  PDF, 287K

• Supplemental file 2 -

  Movie S1. PhnD antibodies inhibit S. epidermidis 1457 biofilm formation.

  AVI, 5.2M

• Supplemental file 3 -

  Movie S2. PhnD antibodies inhibit S. epidermidis 1457-FL biofilm formation when administered in prophylactic mode.

  AVI, 7.5M

• Supplemental file 4 -

  Movie S3. PhnD antibodies inhibit S. epidermidis 1457-FL biofilm formation when administered in early therapeutic mode.

  AVI, 11M

• Supplemental file 5 -

  Movie S4. Deletion of phnD results in defective biofilm formation.

  AVI, 10M

• Supplemental file 6 -

  Movie S5. PhnD antibodies enhance the opsonophagocytosis of S. epidermidis biofilms.

  AVI, 11M

• Supplemental file 7 -

  Movie S6. Single neutrophil tracking of biofilm opsonophagocytosis.

  AVI, 1.3M

• Supplemental file 8 -

  Movie S7. PhnD antibodies inhibit S. epidermidis 1457-FL biofilms grown in human plasma.

  AVI, 7.2M