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Table S1. Primers used in this study. Table S2. List of putative targets up- and downregulated in the sRNA mutant strains identified by proteomic analysis. Fig. S1. Schematic view of the studied sRNAs. Fig. S2. Bacterial persistence within mouse organs in systemic infection. Fig. S3. The E. faecalis wild-type, the ∆ef0408-0409 sRNA mutant, and the complemented strains were grown in GM17 medium supplemented by 1.5 mM H2O2 with shaking (A), in cc17MOPS medium supplemented by glycerol with shaking (B), or in GM17 medium supplemented by 8% NaCl (C) or by 0.08% of bile salts (D), and optical density at 600 nm was determined. Fig. S4. The E. faecalis wild-type, the ∆ef0605-0606 sRNA mutant, and the complemented strains were grown in GM17 medium supplemented by 1.5 mM H2O2 with shaking (A), in cc17MOPS medium supplemented by glycerol with shaking (B), and in GM17 medium supplemented by 0.08% of bile salts (C), and optical density at 600 nm was determined. Fig. S5. The E. faecalis wild-type, the ∆ef1368-1369 sRNA mutant, and the complemented strains were grown in GM17 medium supplemented by 8% NaCl (A) or in horse serum (B), and optical density at 600 nm was determined. Fig. S6. The E. faecalis wild-type, the ∆ef3314-3315 sRNA mutant, and the complemented strains were grown in GM17 medium supplemented by 0.08% of bile salts, and optical density at 600 nm was determined. Fig. S7. Quantitative assays of AP activity under osmotic, oxidative, detergent, and acid stress conditions of transcriptional fusion constructions. Fig. S8. Western blot analysis with polyclonal antibody against Ers (A) and GroEL (B) of E. faecalis wild type (A1 and B1), ∆ef0408-409 mutant (A2 and B2), ∆ef0605-0606 mutant (A3), and ∆ef3314-3315 mutant (B3).
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