Conserved Oligopeptide Permeases Modulate Sporulation Initiation in Clostridium difficile

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    File S1. Vector construction. Fig. S2. Transcriptional analysis of the app and opp operons in wild-type, appA, and oppB null mutants and in a CD0852 (scoC) overexpression strain. Fig. S3. Confirmation of targeted disruption of oligopeptide transporter genes. Fig. S4. Complementation of oligopeptide transporter mutants. Fig. S5. Transcriptional analysis of opp and app expression in complementation studies. Fig. S6. Effects of oligopeptide transporters on regulation of CodY- and CcpA-dependent gene expression. Fig. S7. Total number of all viable cells (vegetative cells and spores) throughout stationary-phase growth.

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