Granelli-Piperno et al. 10.1073/pnas.0402431101. |
Fig. 6. Immature DCs (iDCs) were infected with BaL or influenza virus. After 5 days, HIV-1- and influenza-infected cells were stained for DC-LAMP (lysosome-associated membrane protein) and either HIV-1 p24 or hemagglutinin antigen (HA).
Fig. 7. Sample experiment to detect T cells secreting IFN-gand IL-10. Immature DCs (iDCs) generated from HIV-1-infected patients were treated or not with 1 m M AZT (3’-azido-3’-deoxythymidine) and infected with HIV-1 for 4 days or pulsed at day 3 with 2 mM gag peptides overnight. Other DCs were matured (m) 48 h after infection (Right). DCs were added to autologous T cells and IFN-gand IL-10 release assessed by enzyme-linked immunospot (ELISPOT) assay.