Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie01 - Movie S1. AP dynamics in wild type cells. Real-time imaging of autophagosomes in wild type cells. This time-lapse sequence (12 frames / second) follows the autophagosomes shown in Figure 3, left. The GFP-Atg8 signal is shown in green, and the contour of yeast in brightfield. Note the appearance and disappearance of the GFPAtg8 dots throughout the experiment.
- Movie02 - Movie S2. AP dynamics in vps21Δ mutant cells. Real-time imaging of autophagosomes in vps21Δ mutant cells. This time-lapse sequence (12 frames / second) follows the autophagosomes shown in Figure 3, right. The GFP-Atg8 signal is shown in green, and the contour of yeast in brightfield. Note the accumulation of GFPAtg8 dots after 50 mins.