bio.20148227 Supplementary Material
Files in this Data Supplement:
- Supplementary Material - Loïc Maillard et al. doi: 10.1242/bio.20148227
- Movie 1 - Movie 1. Morphology of SDC4WT-transfected HUV-EC-C. The morphology of SDC4WT-transfected HUV-EC-C was analyzed by live confocal microscopy upon RANTES/CCL5 stimulation for 15 minutes. Membrane protrusions were shown by white arrows. (×400).
- Movie 2 - Movie 2. PKCα translocation of SDC4WT and PKCα -co-transfected HUV-EC-C. The PKCα translocation of PKCα-DsRed2 and GFP-SDC4WT co-transfected HUV-EC-C was analyzed by live confocal microscopy upon RANTES/CCL5 stimulation for 15 minutes. Membrane localization of PKCα (red) was indicated with white arrows. (×400).
- Movie 3 - Movie 3. PKCα translocation of S179A and PKCα -co-transfected HUV-EC-C. The PKCα translocation of PKCα-DsRed2 and GFP-S179A-SDC4 co-transfected HUV-EC-C was analyzed by live confocal microscopy upon RANTES/CCL5 stimulation for 15 minutes. Membrane localization of PKCα (red) was indicated with white arrows. (×400).
- Movie 4 - Movie 4. PKCα translocation of L188QQ and PKCα -co-transfected HUV-EC-C. The PKCα translocation of PKCα-DsRed2 and GFP-L188QQ-SDC4 co-transfected HUV-EC-C was analyzed by live confocal microscopy upon RANTES/CCL5 stimulation for 15 minutes. (×400).