Performance of the Cas9 Nickase System in Drosophila melanogaster

Supporting Information for Ren et al., 2014

Files in this Data Supplement:

• Supporting Information - Figures S1-S6 and Tables S1-S3 (PDF, 2.7 MB)
• Table S3 - Sequences of the oligonucleotides for off-target effect analysis (PDF, 74 KB)
• Figure S1 - Models of Cas9 nuclease or Cas9^H840A nickase in introducing DSBs in DNA (PDF, 1.4 MB)
• Figure S2 - Full-length sequence of the nos-Cas9^D10A (or nos-Cas9^H840A) plasmid(PDF, 97 KB)
• Figure S3 - No significant improvement of survival and fertile G0 rates when applying Cas9 nickase (PDF, 720 KB)
• Figure S4 - No off-target effect detected using the transgenic Cas9 nickase flies to generate heritable mutations (PDF, 185 KB)
• Figure S5 - Representative sequencing results at the break points of the piwi^HDR-mCherry mutant lines (PDF, 432 KB)
• Figure S6 - No off-target effect detected using the CRISPR system to generate piwi mutations (PDF, 206 KB)
• Table S1 - Sequences of the oligonucleotides for mutation detection and vector construction in this study (PDF, 61 KB)
• Table S2 - Sequences of the oligonucleotides for off-target effect analysis (PDF, 62 KB)