Files in this data supplement:
- Supporting Information: Figures S1-S7, Files S1-S2, Tables S1-S3, and References (PDF, 3 MB)
- Figure S1: Evaluation of candidate regulators of the filamentous growth MAPK pathway by the plate-washing assay and cross-talk reporter (FUS1-HIS3). (PDF, 983 KB)
- Figure S2: The role of filamentous growth MAPK pathway regulators in filament formation by the single cell invasive growth assay. (PDF, 264 KB)
- Figure S3: Suppression of hyper-invasive growth phenotypes of mutants identified in the screen by deletion of STE12. (PDF, 275 KB)
- Figure S4: Role of Ras2p and Tpk2p in conditional regulation of the filamentous growth MAPK pathway. (PDF, 166 KB)
- Figure S5: Specificity of filamentous growth MAPK pathway regulators in the mating pathway. (PDF, 1 MB)
- Figure S6: Specificity of filamentous growth MAPK pathway regulators in the HOG pathway. (PDF, 320 KB)
- Figure S7: The filamentous growth MAPK pathway does not regulate the RTG response. (PDF, 241 KB)
- File S1: Serial Z-stack images of rhodamine phalloidin stained wild-type cells grown for 16h in S-GLU. (.mov, 28 KB)
- File S2: Serial Z-stack images of rhodamine phalloidin stained MSB2* cells grown for 16h in S-GLU. (.mov, 77 KB)
- Table S1: Analysis of invasive growth mutants for a role in filamentous growth MAPK pathway regulation. (.xlsx, 70 KB)
- Table S2: Analysis of invasive growth and colony morphology. (.xlsx, 263 KB)
- Table S3: Activity of the FRE-lacZ reporter in mutants that show hyper-filamentous growth. (See sheet 2 for FRE-lacZ analysis of hypo-filamentous growth mutants). (.xlsx, 47 KB)