Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans

Files in this data supplement:

  • Supporting Information: Figures S1-S3, File S1, Table S1, and References (PDF, 242 KB)
  • Figure S1: Schema of gRNA constructs that succeeded or failed to produce the expected phenotype. (PDF, 179 KB)
  • Figure S2: Observations of CRISPR/Cas9 effects on the selected and nonselected alleles. (PDF, 122 KB)
  • Figure S3: Guide RNA cloning strategy. (PDF, 125 KB)
  • File S1: Supplement (PDF, 156 KB)
  • Table S1: Oligonucleotides used in this study. (PDF, 153 KB)