Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie07 - Video 7: Dividing MDCK cells expressing CHMP6‐N‐11‐52‐GFP together with mCherry‐tubulin. Cells were imaged using a spinning disk confocal at 7 minutes intervals. Shown are maximal intensity projections. CHMP6‐N‐11‐52‐GFP, green; tubulin, red.
- Movie09 - Video 9: Dividing MDCK cells expressing CHMP6‐N‐1‐42‐GFP together with mCherry‐tubulin. Cells were imaged using a spinning disk confocal at 7 minutes intervals. Shown are maximal intensity projections. CHMP6‐N‐1‐42‐GFP, green; tubulin, red.
- Movie05 - Video 5: Dividing MDCK cells expressing CHMP6‐N‐GFP together with mCherry‐tubulin. Cells were imaged using a spinning disk confocal at 7 minutes intervals. Shown are maximal intensity projections. CHMP6‐N‐GFP, green; tubulin, red.
- Movie 06 - Video 6: Dividing MDCK cells expressing CHMP6-N-mut-GFP together with mCherry-tubulin. Cells were imaged using a spinning disk confocal at 7 minutes intervals. Shown are maximal intensity projections. CHMP6-N-mut-GFP, green; tubulin, red.
- Movie08 - Video 8: Dividing MDCK cells expressing CHMP6‐N‐11‐42‐GFP together with mCherry‐tubulin. Cells were imaged using a spinning disk confocal at 7 minutes intervals. Shown are maximal intensity projections. CHMP6‐N‐11‐42‐GFP, green; tubulin, red.
- Movie01 - Video 1: Dividing MDCK cells expressing low levels of mCherry‐CHMP6 together with GFP‐tubulin. Cells were imaged using a spinning disk confocal at 7 minutes intervals. Shown are maximal intensity projections. CHMP6, green; tubulin, red.
- Movie04 - Video 4: Dividing MDCK cells expressing low levels of mCherry‐VPS22 together with GFP‐tubulin. Cells were imaged using a spinning disk confocal at 7 minutes intervals. Shown are maximal intensity projections. VPS22, green; tubulin, red.
- Movie02 - Video 2: Dividing MDCK cells expressing low levels of GFP‐VPS36 together with mCherry‐tubulin. Cells were imaged using a spinning disk confocal at 5 minutes intervals. Shown are maximal intensity projections. VPS36, green; tubulin, red.
- Movie 03 - Video 3: Dividing MDCK cells expressing low levels of GFP-GPS36 together with mCherry-tubulin. Cells were imaged using a spinning disk confocal at 5 minutes intervals. Shown are maximal intensity projections. VPS36, green; tubulin, red.